LED不同光强对油茶苗生理生化特性的影响

Effects of different LED intensities on the physiological and biochemical characteristics of oil-tea camellia seedlings

关键词:  经济林学, 育苗, 可溶性物质, 内源激素, 抗氧化酶

Key words:  nonwood forest science, seedling, soluble matter, endogenous hormone, antioxidant enzyme

摘要:

该文以两年生长林4号油茶扦插苗为材料,测定和分析了LED红蓝19复合光不同光强处理下油茶苗可溶性物质含量、内源激素水平及抗氧化酶活性,探索了LED不同光强处理下油茶苗生理生化特性的差异及变化规律。结果表明:100 μmol·m^-2·s^-1处理下油茶苗具有最高的ZR含量和POD活性,以及最低的MDA含量,但可溶性糖含量、IAA含量和GA含量最低; 150 μmol·m^-2·s^-1处理下油茶苗具有最高的ABA含量,且可溶性蛋白含量和SOD活性最低; 200 μmol·m^-2·s^-1处理下油茶苗具有最高的SOD活性,但POD活性最低,且MDA含量最高; 250 μmol·m^-2·s^-1处理下油茶苗具有最高的可溶性蛋白含量、可溶性糖含量、游离氨基酸含量、IAA含量、GA含量和CAT活性; 300 μmol·m^-2·s^-1处理下油茶苗游离氨基酸含量、ABA含量、ZR含量和CAT活性均最低。与其他处理相比,250 μmol·m^-2·s^-1处理更利于提高长林4号油茶苗各项生理生化指标,是培育油茶苗较为理想的光强。

Abstract:

The soluble matter content, endogenous hormone level and antioxidant enzyme activity of oil-tea camellia seedlings treated with different light intensities of LED composite light(10% red lights + 90% blue lights)were mea-sured and analyzed, and the difference and change rules of physiological and biochemical characteristics of oil-tea camellia seedlings under different LED light intensities were explored by taking two-year-old cutting seedlings of ‘Changlin-4' Camellia oleifera as test materials. The results were as follows: Under the light intensity of 100 μmol·m^-2·s^-1, ZR content and POD activity of oil-tea camellia seedlings were the highest, while MDA content was the lowest, but the content of soluble sugar, IAA and GA were also the lowest; Under the light intensity of 150 μmol·m^-2·s^-1, ABA content of oil-tea camellia seedlings was the highest, furthermore, the soluble protein content and SOD activity were the lowest; Under the light intensity of 200 μmol·m^-2·s^-1, SOD activity of oil-tea camellia seedlings was the highest, but POD activity was the lowest, furthermore, the MDA content was the highest; Under the light intensity of 250 μmol·m^-2·s^-1, the soluble protein content, soluble sugar content, free amino acid content, IAA content, GA content and CAT activity of oil-tea camellia seedlings were all the highest; Under the light intensity of 300 μmol·m^-2·s^-1, the free amino acid content, ABA content, ZR content and CAT activity of oil-tea camellia seedlings were all the lowest. Compared with other treatments, 250 μmol·m^-2·s^-1 LED composite light(10% red lights + 90% blue lights), which is more conductive to improve the physiological and biochemical indexes of ‘Changlin-4' C. oleifera seedlings, is the ideal light intensity to cultivate seedlings of oil-tea camellia.