| 摘要: |
| 该文采用硅胶(快速脱水)和35 ℃电子恒温干燥箱中脱水(缓慢脱水)、冷藏(4 ℃)和冻藏(-4 ℃)的方法,研究了辽东栎种子的脱水和低温敏感性及其对种子萌发的影响。结果表明:辽东栎种子成熟散落时具有较高的含水量(96.0%)和萌发率(78.9%),无论是快速脱水还是缓慢脱水,萌发率、萌发速率、萌发指数和活力指数均随着脱水时间的延长和含水量的降低呈现减小的趋势,至脱水96 h后(快速和缓慢脱水种子含水量分别为66.0%和69.8%),种子全部失去活力。辽东栎种子活力与含水量呈显著的正相关关系,但轻度脱水可促进种子萌发,快速脱水种子比缓慢脱水种子具有更高的脱水耐性。辽东栎种子不能耐受低温贮藏,萌发率、萌发速率、萌发指数和活力指数在4 ℃下冷藏30 d或-4 ℃下冻藏6 h后均显著降低,冷藏90 d或冻藏48 h后种子全部丧失活力。 |
| 关键词: 辽东栎, 脱水耐性, 低温贮藏, 种子活力 |
| DOI：10.11931/guihaia.gxzw201809042 |
| 分类号:Q948.1 |
| 文章编号:1000-3142(2019)12-1691-11 |
| Fund project:国家自然科学基金(31660195); 宁夏回族自治区重点研发计划重点项目(2018BEG02001)[Supported by the National Natural Science Foundation of China(31660195); Key Program of Key Research and Development Program of Ningxia Hui Autonomous Region, China(2018BEG02001)]。 |
|
| Recalcitrance of Quercus wutaishanica seeds—Sensitivity to desiccation and low temperature |
|
CHENG Jiming, YAN Xingfu*
|
|
Key Laboratory of Ecosystem Modeling and Applications of State Nationalities Affairs Commission, College of
Biological Science and Engineering, Beifang Minzu University, Yinchuan 750021, China
|
| Abstract: |
| Experiments of desiccation treated by silica gel(rapid desiccation)and electric constant temperature at 35 ℃ drying oven(slow desiccation)and cold(4 ℃)and frozen storage(-4 ℃)were conducted to investigate the sensitivity of Quercus wutaishanica seeds to desiccation and low temperature and their effects on the seed germination. The results showed that high water content(96.0%)and germination percentage(78.9%)of Q. wutaishanica seeds at shedding period. The germination percentage(GP), germination rate(GR), germination index(GI)and vigor index(VI)of seeds desiccated rapidly and slowly all exhibited trends of decline, with the prolonging of desiccation time and the decreasing of seed water content. Desiccation treatment of 96 h(the water content of seeds desiccated rapidly and slowly was 66.0% and 69.8%, respectively)led to entire loss of seed viability. A significantly positive relationship was detected between the seed viability and water content of Q. wutaishanica seeds, while slight desiccation facilitated the seed germination. Seeds desiccated rapidly had higher tolerance to drying compared with seeds desiccated slowly. Seeds of Q. wutaishanica could not tolerate low temperature storage and the GP, GR, GI and VI all markedly declined after being coldly storage at 4 ℃ for 30 d or frozenly storage at -4 ℃ for 6 h. Cold storage at 4 ℃ for 90 d or frozen stored at -4 ℃ for 48 h resulted in that all seeds lost their viabilities. |
| Key words: Quercus wutaishanica, desiccation tolerance, low temperature storage, seed viability |
