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香蕉束顶病和花叶心腐病的快速测定技术研究
周广泉; 邹琦丽; 蒋冬荣; 周志权; 廖咏梅;
1.广西植物所究所;2.广西植物所究所 桂林 541006;3.桂林 541006
摘要:
种植无病蕉苗是防治香蕉束顶病和花叶心腐病的根本措施。筛选无病蕉苗的快速测定技术。除可引用2,3,5-氯化三苯基四(氨)唑浸渍徒手切片;在36℃条件下。24小时后:束顶病株叶脉切片的维管束呈现红色,其它组织为红褐色;花叶心腐病株叶脉切片(含维管束)全部呈现黑褐色;而健株叶脉切片由原绿色变成红色,最后褪成无色。此外。也可取叶片主脉的徒手切片,用1%曙红B水溶液染色。健株切片呈桔红色,病株切片呈砖红色,通过镜检:束顶病株叶片切片的表皮下,可观察到围绕在维管束周围成堆的分布有畸形叶绿体细胞团;花叶心腐病的畸形叶绿体细胞团,多分布在薄壁细胞中,且多散生;而健株的切片没有发现这种畸形的叶绿体细胞团,两种方法同样可靠。
关键词:  香蕉束顶病  香蕉花叶心腐病  2, 3.5-氯化三苯基四(氮)唑  曙红B  
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