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BA对大麦花药培养中药壁的衰退和植株再生频率的影响
钟华鑫; 周菊华; 张飞联; 梁海曼;
1.杭州大学生物系;2.杭州大学生物系 杭州 310012;3.杭州 310012
摘要:
用含20ppm 6-BA的0.1%吐温-80溶液喷施花粉为单核前期的大麦上部叶片和穗部,明显影响大麦花药培养效率。实验结果表明:1)BA处理可明显延缓培养花药的药壁衰退进程。2)BA处理后的花药,在培养期间,其死亡的花粉数比对照大大减少,相反其双核或多核的花粉数比对照明显增加。3)BA处理虽然没有促进大麦花粉愈伤组织的诱导率,但显著地促进愈伤组织的生长。提高愈伤组织成长率,增加可转入分化培养的愈伤组织块数。4)BA处理促进愈伤组织的再分化,尤其是绿苗的分化。
关键词:  大麦  花药培养  BA预处理  绒毡层  愈伤组织诱导和分化  
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