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甘蔗幼叶脱分化及愈伤组织形成过程中内源细胞分裂素和ABA的变化
李军生; 李春瑶; 梁倩华; 杨继华;
1.广西工学院轻纺系;2.广西师范大学生物系;3.广西师范大学生物系 柳州 545005;4.桂林
摘要:
甘蔗幼叶片,叶鞘和幼茎在含有2.4-D的培养条件下脱分化的情况有明显差异。它们除了在形态学上有区别外,这三种材料原有的细胞分裂素和ABA的水平明显不同。在幼叶片脱分化及愈伤组织形成过程中,内源细胞分裂素和ABA的水平也发生了明显变化。据此我们认为在甘蔗组织培养中2.4-D可能通过调节内源激素的水平及其相互作用,引起培养物中某些生理生化过程发生改变,从而进行脱分化和愈伤组织形成。
关键词:  甘蔗  组织培养  内源激素  玉米素  玉米素核苷  
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