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成熟和褐变荔枝果实呼吸作用和脂氧合酶活性
孙谷畴;   
中国科学院华南植物研究所 广州 510650
摘要:
荔枝果实完全成熟和果皮变鮮红时,呼吸速率降低,仅相当于果皮带绿时的39.4%。此时果皮和果肉的脂氧合酶活性亦明显降低,分别相当于后者的60.2%和49.1%。成熟荔枝果实果皮呼吸作用对KCN抑制敏感。2mM KCN抑制果皮总呼吸的91.8%,而仅抑制果肉的56.9%。荔枝果皮呼吸的电了传递主要是通过细胞色素氧化酶途径,而果肉則可能一半是通过其它氧化酶途径。2mKCN和1.5mM SHAM抑制成熟果皮总呼吸97.9%,为SHAM抑制的交替途径呼吸占总呼吸5.28%。相同浓度KCN和SHAM抑制褐变果皮总呼吸79.7%,则SHAM抑制的交替途径呼吸占27.1%。果实褐变时,果成交替途径呼吸比例增高。这一变化可能促进H2O2积累、乙烯产生和果皮褐变深化。
关键词:  荔枝果实褐变  交替途径呼吸  脂氧合酶  
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