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酶解压片分离沙田柚胚襄与胚胎发育研究
薛妙男; 陈腾土;
1.广西师范大学生物系;2.广西柑桔研究所
摘要:
用2.5%纤维素酶与2.5%果胶酶混合液在30℃下对已固定的沙田柚(Citrusgrandisvar.shatinyuHort.)胚乳酶解6—10小时,分离了开花前、授粉至受精间隔期、合子休眠期、胚和胚乳发育各期的胶囊.用Olympas相差显微装置进行观察与摄影,在胚囊中显示了沙田柚胚胎发育各主要阶段的结构特征。分析了酶解压片技术在柑桔属植物胚胎发育研究的前景
关键词:  沙田柚  酶解压片法  胚胎发育  
DOI:
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