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愈伤组织继代接种量、继代周期和年龄对软紫草原生质体脱壁的影响
王黎; 张治国; 王平; 梁海曼;
1.浙江省医学科学院;2.杭州大学生物系
摘要:
本文的实验结果表明:软紫草愈伤组织脱壁所需的适宜酶浓度为:0.1%果胶酶十0.25%纤维素酶;酶解处理的适宜时间与愈伤组织年龄有关:愈伤组织的适宜年龄随其继代周期、愈伤组织继代接种量而有变化。当接种量,1克/瓶,转代后7天进行原生质体分离:接种量3克/瓶,酶解材料则以培养5天愈伤组织为宜。继代周期13天和15天的比15天和15天的,适宜脱壁的愈伤组织当代培养天数要提前1天。
关键词:  软紫草  愈伤组织  原生质体  脱壁  年龄  继代周期  
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