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香蕉试管苗变异因素的初步研究
张海保; 朱西儒; 张云开; 刘卫; 王正询;
1.中国科学院华南植物研究所;2.广州师范学院生物系
摘要:
本文初步研究了香蕉离体培养中,培养基内不同激素浓度、继代培养数,以及3个品种(品系)对试管苗变异的影响。结果表明:631和台湾8号比威廉斯(Williams)稳定,培养条件相同,培养代数多时,变异率明显提高。外源激素的配比试验表明:IBA∶BA为0.5∶4.0(mg/L)为最佳配方,当IBA∶BA达到2.0∶10(mg/L)时,异常苗发生率最高。用田间变异株根尖细胞染色体观察结果,发现其染色体数不是增多就是严重缺失。变异株的过氧化物酶同工酶酶谱经电泳分析,表明在迁移率为0.7处的一条带纹消失,而正常对照苗则稳定存在。
关键词:  香蕉试管苗  变异因素  染色体  同工酶  
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