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红江橙体胚发生及影响因素的研究
贺红; 潘瑞炽; 韩美丽; 李耿光;   
1.华南师范大学生物系;2.中国科学院华南植物研究所
摘要:
红江橙花后4周的幼果,直径约1cm左右,这时期的胚珠最适于胚性愈伤组织的诱导。以MT为基本培养基,加上适当浓度的IAA、BA和ME可显著提高胚性愈伤组织诱导率。体胚发生,以MT+IAA0.1mg1-1+BA(ZT)1mg1-1培养基较好,能诱导产生正常体胚,且频率较高。胚性愈伤组织继代次数对体胚发生也有一定的影响,继代6次以内,胚性愈伤组织具有旺盛产生体胚能力;继代至第9次时,产生体胚的能力明显下降;至第12代时,不能产生胚状体。成熟胚转换成小植株,以MT+GA2mg1-1+NAA0.1mg1-1培养基成苗率最高。
关键词:  红江橙  胚性愈伤组织  体胚发生  
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