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芋茎尖离体培养和快速繁殖
毕可华; 赵建萍; 蒋小满; 柏新付;   
1.烟台师范学院生物系!烟台;264025
摘要:
对芋优良品种莱阳毛羊头进行茎尖培养和快速繁殖。试验表明,外植体表面灭菌的最佳方案是乙醇→新洁尔灭→剥幼叶→升汞。在较软的培养基中,茎尖大于0.6mm,成活率为90%以上。不定芽分化的最适培养基为MS+BA1(mg/L以下单位相同)+NAA0.2+Spm20,其分化率为100%。不定芽在MS+BA2+NAA0.1+Spm50的培养基中,可实现继代繁殖和诱导生根一步完成,月增殖系数达7.3,生根率88.3%。芋试管苗移栽于沙壤土基中,成活率为90%以上,且生长健壮,根系发达。
关键词:  芋  茎尖培养  快速繁殖  
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