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枸杞髓组织离体培养及高频率植株再生的研究
曹有龙; 陈放; 罗青; 曲琳;   
1.四川大学生物系!四川成都610064;2.宁夏农科院农业生物技术研究室!宁夏银川750002
摘要:
枸杞髓组织在4种MS培养基上都能诱导出愈伤组织,诱导率53.7%~100%。在培养基MS+6-BA0.1mg/L+NAA0.5mg/L获得的愈伤组织,呈颗粒状,分散性能好,胚性细胞多.将其转移到MS+6-BA0.5mm/L+NAA0.01mg/L的分化培养基上获得大量绿色小芽,小芽在MS+6-BA0.2mg/L的培养基上得到快速繁殖,繁殖系数50~150株/芽·月。丛生芽在MS+NAA0.2tmg/L的培养基上形成完整植株
关键词:  枸杞  髓组织  离体培养  植株再生  
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