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大蒜愈伤组织诱导条件初报
李志勇; 郭勇; 罗焕亮;   
1.华南理工大学食品与生物工程学院!广东广州1510641;2.广州市林业科学研究所!广东广州510515
摘要:
以白皮大蒜和大头蒜为材料,报道了大蒜品系、外植体的低温前处理、外植体大小及取材种类不同对愈伤组织诱导的影响。结果表明:(1)无论是4℃低温保藏或室温保存前处理,大头蒜的消毒效果均优于白皮大蒜,4℃低温前处理可降低外植体出愈污染;(2)只有带表皮的外植体才可诱导愈伤组织,外植体较大(0.5cm×0.3cm×0.2cm),继代期愈伤组织产生量较多;外植体偏小(0.2cm×0.1cm×0.1cm),极少产生愈伤组织;(3)4℃冷藏18d对大头蒜愈伤组织诱导有一定影响,但对白皮大蒜影响不大。冷藏可推迟出愈1~2d。
关键词:  大蒜  愈伤组织  外植体  低温处理  
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