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五种黄精属植物的蛋白水解酶谱研究
张为民; 张钧; 邢智峰; 姬生栋; 胡轶红; 徐存拴;
1.焦作教育学院生物系!河南焦作483005;2.河南师范大学生物系!河南新乡453002
摘要:
用蛋白水解酶复性电泳方法 (G- PAGE)分析了 5种黄精属植物根状茎和叶的蛋白水解酶的种类和活性。结果表明 :(1)它们的根状茎均含有 85k D和 55k D的蛋白水解酶 ;叶均含有 82 k D的蛋白水解酶 ;(2 )根状茎和叶的蛋白水解酶种类和活性有很大差异 ,叶的蛋白水解酶活性为根状茎的 10倍 ,它们的活性均受 p H影响 ,其最适 p H为 7;(3)每种植物都含有自己特有蛋白水解酶 ;(4 )蛋白水解酶在植物鉴定中有参考价值
关键词:  黄精属植物  蛋白水解酶  蛋白水解酶复性电泳  
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