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枸杞髓组织培养中体细胞胚胎发生与过氧化物酶同工酶分析
魏琴; 曹有龙; 陈放; 周黎军; 陈东林;   
1.宜宾师范高等专科学校生物系!四川宜宾644007;2.四川大学生命科学学院!四川成都610064;3.宜宾农业学校!四川宜宾644003
摘要:
枸杞髓组织在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L培养基上诱导愈伤组织发生。在 MS+6 - BA0 .1mg/ L+NAA0 .5mg/ L+CH50 0 mg/ L培养基上继代培养 ,再转入 MS+6 - BA2 mg/L +NAA 0 .5mg/ L的分化培养基上进行分化培养。显微观察表明 ,在培养过程中愈伤组织细胞由非胚性细胞转变为胚性细胞 ,直至发育成体细胞胚胎和完整植株 ;电泳结果显示 ,体细胞胚胎发生的各阶段 ,其过氧化物酶同工酶发生相应的变化。
关键词:  枸杞  髓组织培养  体细胞胚胎  过氧化物酶同工酶  
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