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毛白杨愈伤组织悬浮细胞耐盐性研究
赵素然; 梁宇; 李德森; 王颖; 杜荣骞;
南开大学生命科学学院!天津300071
摘要:
把生长在全盐含量为 0 .361%的土壤中的毛白杨 ( Populus tomentosa Carr.)愈伤组织悬浮细胞培养在含不同浓度 Na Cl的液体培养基中 ,定期测量各组的细胞密度 ,根据生长曲线研究其耐盐性。结果显示 ,与不加 Na Cl的液体培养基相比 ,生长于含较低浓度 Na Cl( 2 .7‰和 5.5‰ )液体培养基中的细胞生长较好 ,而在含较高浓度 Na Cl( 8.2‰和 10 .9‰ )的液体培养基中 ,细胞生长受到较强抑制。
关键词:  毛白杨  耐盐性  愈伤组织  悬浮细胞培养  生长曲线  
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