| 摘要: |
| 该文以青钱柳幼嫩茎段为外植体,研究其种苗快速繁殖技术。结果表明:青钱柳最佳采样时间为4月—6月,最佳外植体为轻微木质化茎段; 最佳的外植体表面消毒方法为用0.1% HgCl2浸泡5～7 min,消毒成功率54.1%,无菌外植体存活率88.7%; 初代芽诱导培养基为MS+6-BA 2.0 mg 瘙 簚 L-1+IBA 0.2 mg 瘙 簚 L-1+蔗糖30.0 g 瘙 簚 L-1,芽诱导率80.5%,培养21 d初代芽苗平均高度3.0 cm; 最佳继代增殖培养基为MS+6-BA 0.5 mg 瘙 簚 L-1+IBA 0.05 mg 瘙 簚 L-1+TIBA 0.02 mg 瘙 簚 L-1+蔗糖30.0 g 瘙 簚 L-1,培养35 d,增殖系数7.0/35 d,平均苗高4.5 cm,芽苗健壮且无玻璃化; 生根前的壮苗培养基为MS+6-BA 0.5 mg 瘙 簚 L-1+IBA 0.05 mg 瘙 簚 L-1+蔗糖30.0 g 瘙 簚 L-1,培养35 d,长出的芽苗高大健壮,平均苗高6.0 cm; 生根培养基为1/2WPM+IBA 1.5 mg 瘙 簚 L-1+5-NGS 4.5 mg 瘙 簚 L-1+蔗糖20.0 g 瘙 簚 L-1,培养40 d,最高生根率83.3%; 生根苗较适合的移栽基质为泥碳土,较好的移栽季节为3月—5月和10月—11月,移栽后在遮光度70%的大棚中培养40 d,移栽成活率为54.3%～65.6%。该研究为其优良无性系的规模化繁育奠定基础。 |
| 关键词: 青钱柳, 幼嫩茎段, 快速繁殖 |
| DOI：10.11931/guihaia.gxzw201808014 |
| 分类号:Q943 |
| 文章编号:1000-3142(2020)01-0108-11 |
| Fund project:广西科技创新能力与条件建设计划项目(桂科能1598025-36); 广西林业科技项目(桂林科研[2015]第28号); 广西喀斯特植物保育与恢复生态学重点实验室自主课题项目(19-050-06); 国家自然科学基金(31160055)[Supported by the Program of Guangxi Scientific and Technological Innovation Capacity and Conditions Construction Plan(1598025-36); Program of Guangxi Forestry Science and Technology( [2015] No. 28); Fund of Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain(19-050-06); the National Natural Science Foundation of China(31160055)] 。 |
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| Rapid propagation technology of Cyclocarya paliurus |
|
HUANG Ningzhen, SU Jiang, XIAN Kanghua, FU Chuanming,
GONG Qingfang, HUANG Huijin, HE Jinxiang*
|
|
Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany,
Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
|
| Abstract: |
| Cyclocarya paliurus is a unique fast-growing tree in China and a mono species of Cyclocarya genus in Juglandaceae family. It has important medical value and is honored as “the third tree in the medical field”. But its seedling breeding is difficult and inefficient. Therefore, tissue culture and rapid propagation of Cyclocarya paliurus were studied by using the young stem segments as explants. The results were as follows: The optimal sampling time of explants was from April to June. The best explants for tissue culture were the slight lignification stem segments. And the best explants surface disinfection method was to soak stem segments in 0.1% HgCl2 for about 5-7 min. The success rate of disinfection was 54.1% and the survival rate of aseptic explants was 88.7%. The medium of MS+6-BA 2.0 mg 瘙 簚 L-1+IBA 0.2 mg 瘙 簚 L-1+sugar 30.0 g 瘙 簚 L-1 was suitable for primary bud induction culture, and the bud induction rate was 80.5%. After 21 d cultivation, the average height of primary buds was 3.0 cm. The best subculture medium for multiplication was MS+6-BA 0.5 mg 瘙 簚 L-1+IBA 0.05 mg 瘙 簚 L-1+TIBA 0.02 mg 瘙 簚 L-1+sugar 30.0 g 瘙 簚 L-1. After 35 d of cultivation, the proliferation coefficient was 7.0/35 d, and the shoots were strong and free of vitrification with an average height of 4.5 cm. The medium for strong shoots before rooting was MS+6-BA 0.5 mg 瘙 簚 L-1+IBA 0.05 mg 瘙 簚 L-1+sugar 30.0 g 瘙 簚 L-1. Cultivated in this medium for 35 d, the shoots grew tall and strong, with an average height of 6.0 cm. The rooting medium was 1/2 WPM+IBA 1.5 mg 瘙 簚 L-1+5-NGS 4.5 mg 瘙 簚 L-1+sugar 20.0 g 瘙 簚 L-1. Cultivated 40 d in this medium, and the highest rooting rate was 83.3%. The suitable transplanting matrix for rooting seedlings was slimy soil, and the best transplanting seasons were the periods from March to May and from October to November. After transplanting, the rooting seedlings were cultured for 40 d in a greenhouse with a shade of 70%, and the survival rates were 54.3%-65.6%. This work would lay a foundation for large-scale breeding of its superior clones. |
| Key words: Cyclocarya paliurus, young stem segments, rapid propagation |
