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药用植物川东獐牙菜的组织培养
黄衡宇;陈义光
1.吉首大学生物系;2.吉首大学生物系 湖南吉首416000;3.云南大学生物系;4.云南昆明650091;5.湖南吉首416000
摘要:
针对川东獐牙菜野生资源受到严重破坏的情况 ,系统地探讨了通过组织培养为手段进行人工繁殖的方法 ,旨在为川东獐牙菜的保护提供坚实的理论依据。研究结果表明 :在所有的实验方案中 ,幼茎和老叶是理想的外植体材料。对叶片来说 ,较适宜的诱导愈伤组织的激素组合是 Zt1.0 m g/L +NAA 0 .0 5m g/L +IBA0 .0 5mg/L、BA 0 .5m g/L+2 ,4 - D0 .5mg/L或 BA 0 .2 m g/L+2 ,4 - D0 .2 mg/L+IBA0 .1m g/L ,对茎段来说 ,较适宜的诱导愈伤组织的激素组合是 BA0 .0 5m g/L+kt0 .0 5mg/L+I...
关键词:  川东獐牙菜    组织培养    愈伤组织    芽丛    生根
DOI:
分类号:
Fund project:湖南省教育厅资助项目 (编号 :0 0 C191)
Abstract:
Key words:  
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