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基因枪介导甘蔗遗传转化几个影响因素的研究
秦新民;叶云;于兰
1.广西师范大学生命科学学院;2.广西师范大学生命科学学院 广西桂林541004;3.广西桂林541004
摘要:
用基因枪GJ1000介导将Bt基因转入甘蔗嫩叶、Ⅰ型愈伤组织和Ⅱ型愈伤组织。结果表明,用Ⅱ型愈伤组织作为受体最用利于转化;气体压力、轰击距离、轰击次数和真空度对转化效率有不同程度的影响。条件优化后,得到了大量的抗性愈伤组织和一些抗性植株,转化率分别为34.9%和3.36%。
关键词:  甘蔗    基因枪    遗传转化    抗性植株
DOI:
分类号:
Fund project:广西自然科学基金资助项目(20007007)
Abstract:
Key words:  
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