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激素对洋桔梗植株再生的影响及生根培养的研究
李群;刘光勇;王丽
1.四川师范大学化学与生命科学学院;2.成都市猛追湾中学;3.四川大学生命科学学院 四川成都610066;4.四川成都610051;5.四川成都610064
摘要:
以MS为基本培养基 ,附加不同浓度的 6 BA、KT、NAA和IBA诱导洋桔梗叶片外植体的再生植株。结果表明 :MS +6 BA 0 .5~ 1 .0mg/L(单位下同 ) +NAA 0 .2和MS +6 BA 0 .5~ 1 .0 +IBA 0 .2培养基都能诱导外植体产生愈伤组织 ,但 6 BA的浓度必须小于 1 .0mg/L ,否则会导致组织的严重玻璃化 ;MS +KT 1 .0~2 .0 +NAA 0 .2或MS +KT 1 .0~ 2 .0 +IBA 0 .2培养基也能诱导外植体产生愈伤组织 ,愈伤组织出现的时间较早且质地较好 ,适合分化。继代培养时 ,MS培养基中仅加 6 BA 0...
关键词:  洋桔梗    激素    植株再生
DOI:
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Fund project:四川省教育厅青年基金项目
Abstract:
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