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高效液相色谱法检测发酵液中木糖和木糖醇
方宏;曾健智;张厚瑞
1.广西壮族自治区中国科学院广西植物研究所;2.广西壮族自治区中国科学院广西植物研究所 广西桂林541006;3.广西桂林541006
摘要:
建立高效液相色谱检测发酵液中木糖和木糖醇含量的分析方法。色谱柱为HypersilNH2 柱 (4 .6mmi.d.× 2 5 0mm ,5 μm) ,柱温 3 5℃ ,流动相为乙腈—水 (80∶2 0 ) ,流速 1 .0mL .min 1,示差折光检测器检测。木糖和木糖醇在 3 .0~ 60mg.mL 1范围内 ,峰面积与其浓度线性关系良好 (г=0 .9995 ) ;平均回收率分别为 96.0 7% (n =5 ,RSD =0 .5 1 % )和 97.47% (n =5 ,RSD =1 .1 3 % )。方法简便、快速、准确。
关键词:  木糖    木糖醇    发酵    高效液相色谱
DOI:
分类号:
Fund project:广西自然科学基金资助项目 (98110 15 )
Abstract:
Key words:  
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