• 1
  • 2
  • 3
  • Home
  • About Journal
  • Editorial Board
    The 8th Editorial Board of Guihaia
    The Previous Editorial Boards of Guihaia
  • Subscription
  • For Authors
    Contact Us
    Writing Guideline
    Submission Guideline
  • Open Access
  • Ethcis Statement
  • Chinese
Cite this article:[Click copy]
[Click copy]
【Print this page】   【Download PDF full text】   【View/Add Comment】  【Download reader】  【 Close 】
←Previous|Next→ Archive     Advanced search
This article has been:browse 7940times   Download 3506times 本文二维码信息
Scan the code!
Share to: WeChat More
Script:Enlarge+|Default|Narrow-
影响农杆菌介导玉米愈伤组织遗传转化因素的研究
农友业;何勇强;覃燕;Rachid Loukehaich;吴子恺
1.广西大学农学院;2.广西大学生命科学技术学院;3.广西大学农学院 广西南宁530005;4.广西南宁530005
摘要:
用农杆菌介导玉米愈伤组织的转化,其筛选的结果得到的抗性愈伤组织受玉米愈伤组织的继代时 间、浸染的农杆菌菌液浓度、共培养的温度以及其共培养时间等因素的影响。玉米愈伤继代后7~9d,农杆菌 浓度为OD600值0.3左右、共培养温度约22℃、培养时间3d时,抗性愈伤的获得率最高。
关键词:  玉米    愈伤组织    农杆菌    转化    影响因素
DOI:
分类号:
Fund project:国家转基因植物研究与产业化专项项目(J00 003 03)
Abstract:
Key words:  
桂ICP备05007876号-3
Adress:Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, No. 85, Yanshan Street, Yanshan District, Guilin, Guangxi, China   Editorial Office of Guihaia,Postal code: Tel/Fax:0773-3550074
E-mail:guihaia@vip.163.com;guihaia@126.com;guihaia@gxib.cn   website:www.guihaia-journal.com
Technology:Beijing Qinyun Science and Technology Development Co., Ltd.