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在培养基中加入抗生素防止万年青茎段培养污染的研究
周俊辉;杨妙贤;李春霞;宋苑芬
1.仲恺农业技术学院园艺系;2.仲恺农业技术学院园艺系 广东广州510225;3.广东广州510225
摘要:
以玛丽安万年青茎段为试验材料,将浓度为50mg·L1的青霉素、氯霉素、利福平、庆大霉素、先锋霉素6号、链霉素和卡拉霉素7种抗生素分别经过过滤灭菌后加入到MS基本培养基,从中筛选出防止污染效果较好的两种抗生素,分别按50、100、150mg·L1的浓度加入到MS培养基中培养,以筛选出最适合浓度;然后再筛选出利福平和氯霉素的最佳浓度组合。结果表明:7种抗生素中以利福平和氯霉素防止污染效果较好,未污染率分别为68.42%和58.33%,成活率分别为65.79%和52.78%,利福平和氯霉素的浓度均为150mg·L1时防止污染的效果最佳;25mg·L1利福平+50mg·L1氯霉素和50mg·L1利福平...
关键词:  玛丽安万年青    抗生素    茎段培养    防止污染
DOI:
分类号:
Fund project:国家星火计划资助项目(2001EA780018)
Abstract:
Key words:  
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