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芦荟属植物种间杂交及其F_1代POD同工酶鉴定
铁军;金山;白海艳;刘瑞祥;吴志萍
1.长治学院生化系;2.内蒙古农业大学林学院 山西长治046011;3.山西长治046011;4.内蒙古呼和浩特010019
摘要:
以芦荟属植物元江芦荟和皂质芦荟为亲本进行远缘有性杂交试验。正交对3株103朵小花进行授粉,获得7枚果实,平均结实率为6.80%;反交对2株88朵小花授粉,获得5枚果实,平均结实率为5.88%。获得了19株F1代实生苗,并对亲本和F1代实生苗以及库拉索芦荟、华芦荟等5种芦荟属植物进行了过氧化物酶同工酶(POD)的比较研究,结果显示,5种芦荟种间的POD同工酶酶谱具有较高的相似程度,同时各种又具有各自的特征酶带,很容易区分。特别是F1代实生苗的酶谱与父母本的酶谱有显著不同的酶带,证明产生了新的中间类型。
关键词:  芦荟属    种间杂交    F1代实生苗    POD同工酶
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