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铁皮石斛快速繁殖和离体种质保存的研究
罗吉凤;程治英;龙春林;
1.中国科学院昆明植物研究所;2.中国科学院昆明植物研究所 云南昆明650204;3.云南昆明650204
摘要:
对铁皮石斛种子发芽、原球茎增殖、丛芽分化和壮苗培育进行了试验、观察和分析,研究了培养基、植物激素、光强和添加剂等对其分化和生长的影响。结果显示种子在1/2MS+蔗糖2%的培养基上,30d萌发95%以上。原球茎在1/2MS+椰子汁25%+蔗糖3%的培养基上,45~60d原球茎增殖速度可达1∶10。丛芽分化较适宜的培养基为1/2MS+BA2mg·L-1+NAA0.2mg·L-1+IBA0.1mg·L-1+蔗糖3%,45~60d芽丛增殖速度为1∶4~5。试管苗在MS+香蕉泥20%+蔗糖2%培养基上,大约60d苗快速长高,茎粗壮且根系发达。离体保存材料可采用试管丛芽和原球茎两种方式,以保持其遗传多样性...
关键词:  铁皮石斛    快速繁殖    离体种质保存
DOI:
分类号:
Fund project:国家科技基础条件平台项目(2004DKA30430);; 中国科学院知识创新工程重要方向项目(KSCX2-SW-117)资助~~
Abstract:
Key words:  
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