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大白菜细胞质雄性不育保持系3411-7——RAPD特异扩增片段的克隆测序
王永飞;马三梅;张鲁刚;
1.暨南大学生物工程学系;2.西北农林科技大学园艺学院 广东广州510632;3.广东广州510632;4.陕西杨陵712100
摘要:
利用RAPD技术从大白菜细胞质雄性不育保持系3411-7的DNA中得到一个特异扩增片段MOPB04600。回收该特异扩增片段并将其克隆到pGEM-TEasy载体上进行序列测定。结果表明该片段全长600bp,其碱基组成为A+T=72.33%。通过与GenBank+EMBL+DDBJ+PDB中的455,972个序列进行同源性比较,同源性均小于30%,表明该片段为一新发现的序列。并对该特异片段的来源及其可能的作用进行探讨。
关键词:  大白菜    细胞质雄性不育    RAPD    克隆    测序
DOI:
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Fund project:暨南大学引进人才启动基金(692017)~~
Abstract:
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