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RP-HPLC测定红丝线中香豆素的含量
蒋小华;谢运昌;黄永林;
1.广西壮族自治区中国科学院广西植物研究所;2.广西壮族自治区中国科学院广西植物研究所 广西桂林541006;3.广西桂林541006
摘要:
采用反相高效液相色谱法,测定红丝线中香豆素的含量。色谱柱为ZORBAXXDB-C18(4.6mm×150mm,5μm);流动相:V(甲醇)∶V(0.01mol/L磷酸二氢钠溶液(pH5.4))=45∶55;流速:1mL/min;检测波长278nm。香豆素的线性范围为2.5~30mg/L(r=0.9998),回收率97.5%~101%。该法简便、准确,重复性好,适用于测定红丝线中香豆素的含量。
关键词:  反相高效液相色谱法    爵床科    红丝线    香豆素
DOI:
分类号:
Fund project:国家自然科学基金(30360117);; 广西自然科学基金(0342002-2);; 国家科技攻关计划课题(2005BA901A10);; (“西部开发”重大项目)资助~~
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