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莲C_0t-1 DNA的荧光原位杂交分析
刁英;佘朝文;胡中立;宋运淳;
1.武汉大学发育生物学教育部重点实验室;2.重庆文理学院生命科学系 重庆402168;3.湖北武汉430072
摘要:
为分析中国莲C_0t-1 DNA在其中期染色体上的分布,从中国莲基因组DNA中分离出C_0t-1 DNA,将基因组和所分离的C_0t-1 DNA用生物素标记后作探针,对中国莲染色体进行原位杂交。杂交结果用耦联有荧光素Cy3的生物素抗体检测,发现在每对染色体上均显示出特定的荧光原位杂交带。同时分析了FISH和GISH信号分布的异同。基于C_0t-1 DNA荧光原位杂交带型及染色体型,构建了中国莲核型。
关键词:  中国莲    C_0t-1 DNA    荧光原位杂交    基因组荧光原位杂交    核型
DOI:
分类号:
Fund project:国家科技攻关项目(2002BA546C);; 湖南省科技攻关重大项目(2002AA205A);; 武汉市科技攻关重点项目(20022005132)~~
Abstract:
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