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无核荔枝果实形成差异表达基因cDNA的克隆
禤维言;郑学勤;
1.中国热带农业科学院热带作物生物技术国家重点实验室;2.中国热带农业科学院热带作物生物技术国家重点实验室 海南海口571107;3.广西大学农学院;4.广西南宁530005;5.海南海口571107
摘要:
采用抑制差减杂交技术(Suppression Subtractive Hybridization,SSH)分离与海南无核荔枝果实形成相关的差异表达基因的cDNA片段,为克隆相关基因提供研究基础。分别以无核荔枝的有核幼果为driver;无核幼果为tester,建立差减cDNA文库。经Reverse Northern Dot-Blot筛选该文库,共获得61个阳性克隆,随机选取17个克隆进行测序,共获得10条非重复序列,对其中较长的7个序列进行同源分析,结果表明:有6个序列在荔枝中为首次报道。
关键词:  无核荔枝    无核果实    抑制差减杂交(SSH)    Reverse Northern Dot-Blot
DOI:
分类号:
Fund project:国家“863”计划项目资助(AA001380)~~
Abstract:
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