| 摘要: |
| TIR-NBS基因是一类与植物抗病调节和生长发育密切相关的基因,其特征解析有助于对植物免疫和发育调节的认识。该研究通过生物信息学分析方法,从基因鉴定、染色体分布、基因结构、蛋白性质和结构、信号肽、亚细胞定位和顺式作用元件等方面,对烟草TIR-NBS基因家族进行了鉴定和分子特性分析。结果表明:烟草TIR-NBS基因家族含有30条成员,分布在21条染色体上,含有3～8个保守基序; 编码蛋白均为不稳定蛋白,无信号肽,主要分布于细胞核、细胞质和叶绿体中,二级结构以α-螺旋和无规则卷曲为主要构成元件; 进化时主要受纯化选择作用,与番茄的TIR-NBS基因亲缘关系最近; 顺式作用元件分析表明其可能受光照、温度以及生长素、茉莉酸甲酯、脱落酸、水杨酸、赤霉素等激素调控。该研究结果为进一步探究TIR-NBS基因的生物学功能及提高植物产量和品质提供了依据。 |
| 关键词: 烟草, 抗病调节, 生长发育, 植物免疫, TIR-NBS基因家族, 生物信息学分析 |
| DOI：10.11931/guihaia.gxzw201909045 |
| 分类号:Q943 |
| 文章编号:1000-3142(2020)06-0891-10 |
| Fund project:国家转基因生物新品种培育科技重大专项项目(2016ZX08010003); 贵州省科技计划项目(黔科合基础 [2017]1044号,黔科合平台人才 [2017]5788号)[Supported by the National Major Program of Genetically Modified Organisms Breeding of China(2016ZX08010003); Guizhou Provincial Program of Science and Technology([2017]1044,[2017]5788)]。 |
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| Bioinformatics analysis of tobacco TIR-NBS gene family |
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DONG Yaping1, YANG Shimei1, ZHAO Degang1,2, SONG Li1*
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1. College of Life Sciences and Institute of Agro-Bioengineering, Guizhou University/Key Laboratory of Plant Resources Conservation
and Germplasm Innovation in Mountainous Region(Ministry of Education)/Guizhou Key Lab of Agro-Bioengineering,
Guiyang 550025, China;2. Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
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| Abstract: |
| TIR-NBS gene is a class of gene closely related to plant disease resistance regulation and growth and development, and its characteristic analysis contributes to the understanding of plant immunity and developmental regulation. Through bioinformatics analysis method, the TIR-NBS gene family of tobacco was identified and molecularly analyzed from the aspects of gene identification, chromosome distribution, gene structure, protein properties and structure, signal peptide, subcellular localization and cis-acting elements. The results were as follows: The TIR-NBS gene family contained 30 members, distributed on 21 chromosomes, containing 3-8 conserved motifs; The encoded proteins were unstable proteins, no signal peptides, mainly distributed in the nucleus, cytoplasm and chloroplast; The secondary structure was characterized by α-helix and random coiling; It was mainly selected by purification in evolution, and had the closest relationship with TIR-NBS gene of tomato; Analysis of cis-acting elements showed that it may be regulated by light, temperature, and hormones such as auxin, methyl jasmonate, abscisic acid, salicylic acid, and gibberellin. The results provided a basis for further exploring the biological functions of the TIR-NBS gene and improving plant yield and quality. |
| Key words: Nicotiana attenuata(tobacco), disease resistance regulation, growth and development, plant immunity, TIR-NBS gene family, bioinformatics analysis |
