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巴戟天组织培养和快速繁殖研究
黄宁珍;付传明;赵志国;唐凤鸾;李锋;
1.广西壮族自治区中国科学院广西植物研究所;2.广西壮族自治区中国科学院广西植物研究所 广西桂林541006;3.广西桂林541006
摘要:
以巴戟天顶芽及嫩茎节段为外植体,以MS为基本培养基,通过不同的激素种类和浓度配比,建立巴戟天组培快繁体系。结果表明,外植体表面消毒以70%酒精预处理60s,再用0.1%HgCl2浸泡10min,效果较好,茎节为外植体优于顶芽。培养基MS+BA1.0mg/L+IBA0.05mg/L利于诱导出芽,可用于初代培养;MS+BA1.0mg/L+IBA0.2mg/L利于形成丛生芽,用于继代增殖,繁殖系数6.0/50d;1/2MS+IBA0.4~0.8mg/L适宜诱导生根获得再生植株,生根率100%;生根苗移栽于排水良好的火土或砂土中,成活率90%。
关键词:  巴戟天    顶芽及嫩茎节段    组织培养与快速繁殖
DOI:
分类号:
Fund project:广西科技攻关项目(桂科攻0322024-3B)~~
Abstract:
Key words:  
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