| 摘要: |
| 以广藿香叶片、带节茎、不带节茎及根尖为材料进行离体培养,对影响离体再生的因素进行了研究。结果表明:BA有利于广藿香外植体出芽,浓度以0.1～0.5 mg/L效果较好; 不同外植体的出芽能力有较大差异,其中以叶片和带节茎出芽能力较强,出芽率均达100%; 外植体在培养基上的接种方式对出芽也有一定影响,带节茎以形态学下端垂直插入,可以缩短出芽时间及增加单个外植体出芽的数量。无根苗生根以MT+IBA0.2 mg/L培养基为好,苗的生长较为健壮。 |
| 关键词: 广藿香 外植体 离体培养 |
| DOI： |
| 分类号:Q943.1 |
| Fund project: |
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| In vitro culture of different explants from Pogostemon cablin |
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LIN Xiao-Hua, HE Hong*, WU Li-Rong,
ZHANG Gui-Fang, ZHANG Yan-Ling
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College of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China
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| Abstract: |
| The leaf segments, nodular stem segments,stem segments and root tips from Pogostemon cablin were cultured in vitro. The factors affecting plant regeneration were studied. The results were as follows:BA was effective to induce shoot formation; the optimal BA concentration for shoot formation was 0.1～0.5 mg/L; there was significant difference on the shoot formation from different explants; the leaf segments and nodular stem segments were the most suitable for culture,with the highest shoot formation frequency of 100%; nodular stem segments oriented with their apical ends protruding from the medium could produce more shoots in less time than when they were placed with their basal end upright or were placed horizontally. Optimal rooting medium for regeneration shoots was MT+IBA 0.2 mg/L. |
| Key words: Pogostemon cablin explant in vitro culture |
