彩色大白菜子叶的不定芽再生

Shoot regeneration from cotyledon of orange-heading Chinese Cabbage

关键词:  彩色大白菜  子叶片  再生

Key words:  Brassica campestris ssp.pekinensis  cotyledon  regeneration

摘要:

以彩色大白菜子叶为外植体,研究不同激素配比和AgNO₃对不定芽再生的影响。结果表明:单独附加细胞分裂素(6-BA或TDZ)的MS培养基,不能诱导子叶不定芽分化; 而同时附加生长素(NAA)和细胞分裂素(6-BA或TDZ),不定芽的再生频率提高,最高为15%; AgNO₃与细胞分裂素及生长素配合使用,能大幅度提高子叶不定芽的再生频率,提高率最高达42.5%。与6-BA相比,TDZ对不定芽再生的效果更好。当TDZ浓度为0.05 mg/L、NAA为0.3 mg/L、AgNO₃为8 mg/L时,产生丛状芽数目最多,再生率最高,达50%。

Abstract:

The effects of hormone combination and AgNO₃ on adventitious shoot regeneration from cotyledon of orange-heading Chinese Cabbage were studied. The results showed that the adventitious shoot could not be induced from the cotyledon in MS medium added with cell division hormone(6-BA or TDZ)only; the regeneration frequency of shoot reached to 15% in MS medium added with cell division hormone(6-BA or TDZ)and growth hormone(NAA)together; the regeneration frequency could increase 42.5% in MS medium added with cell division hormone(TDZ),growth hormone(NAA)and AgNO₃. Compared with 6-BA,TDZ had more effective in inducing shoot regeneration. The maximum frequency of shoot regeneration(50%)was obtained in MS medium which contained 0.05 mg/L TDZ,0.3 mg/L NAA and 8 mg/L AgNO₃.