| 摘要: |
| 以彩色大白菜子叶为外植体,研究不同激素配比和AgNO3对不定芽再生的影响。结果表明:单独附加细胞分裂素(6-BA或TDZ)的MS培养基,不能诱导子叶不定芽分化; 而同时附加生长素(NAA)和细胞分裂素(6-BA或TDZ),不定芽的再生频率提高,最高为15%; AgNO3与细胞分裂素及生长素配合使用,能大幅度提高子叶不定芽的再生频率,提高率最高达42.5%。与6-BA相比,TDZ对不定芽再生的效果更好。当TDZ浓度为0.05 mg/L、NAA为0.3 mg/L、AgNO3为8 mg/L时,产生丛状芽数目最多,再生率最高,达50%。 |
| 关键词: 彩色大白菜 子叶片 再生 |
| DOI： |
| 分类号:Q943.1 |
| Fund project: |
|
| Shoot regeneration from cotyledon of orange-heading Chinese Cabbage |
|
QU Hui-Ling1,2, ZHANG Lu-Gang1*, FAN Ai-Li1
|
|
1.College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100,
China;2.Agricultural Bureau of Yuncheng City, Yuncheng 044000, China
|
| Abstract: |
| The effects of hormone combination and AgNO3 on adventitious shoot regeneration from cotyledon of orange-heading Chinese Cabbage were studied. The results showed that the adventitious shoot could not be induced from the cotyledon in MS medium added with cell division hormone(6-BA or TDZ)only; the regeneration frequency of shoot reached to 15% in MS medium added with cell division hormone(6-BA or TDZ)and growth hormone(NAA)together; the regeneration frequency could increase 42.5% in MS medium added with cell division hormone(TDZ),growth hormone(NAA)and AgNO3. Compared with 6-BA,TDZ had more effective in inducing shoot regeneration. The maximum frequency of shoot regeneration(50%)was obtained in MS medium which contained 0.05 mg/L TDZ,0.3 mg/L NAA and 8 mg/L AgNO3. |
| Key words: Brassica campestris ssp.pekinensis cotyledon regeneration |
