| 摘要: |
| 采用电泳技术结合同工酶染色,分析了罗汉果雌雄株叶片的过氧化物酶同工酶、酯酶同工酶、超氧化物歧化酶同工酶、多酚氧化酶同工酶和过氧化氢酶同工酶。结果表明:罗汉果雌雄叶片在同工酶谱上,存在着与性别性状相关的酶带; 雌雄间的差异酶带在每一种同工酶中均有一条以上,可作为罗汉果雌雄株间的性别鉴定。此外,还比较了高产、低产、不结果雌株之间同工酶的酶带和活性差别。 |
| 关键词: 罗汉果 性别性状 同工酶 雌雄株 |
| DOI： |
| 分类号:Q946.5 |
| Fund project: |
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| Sex identification of Siraidia grosvenorii by isozyme analysis |
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LI Hui-Min1, HUANG Xi-Yang1,2, GAO Cheng-Wei1, QIN Xin-Min1*
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1.College of Life Sciences, Guangxi Normal University, Guilin 541004, China;2. Guangxi Institute of Botany,
Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
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| Abstract: |
| The peroxidase,esterase,superoxide dismutase,polyphenol oxidase and catalase isozyme of Siraidia grosvenorii were analyzed with vertical slab PAGE and specific cytochemical staining. The results showed that isozyme bands have apparent difference between the male and female plant. The number of different band of sex character was above one in each isozyme. It is concluded that the difference on isozyme bands can be used for sex identification of Siraidia grosvenorii. In addition,the isozyme bands and enzyme activity of high yield female plant,low yield female plant and acarpous female plant were compared. |
| Key words: Siraidia grosvenorii sex character isozyme male and female plant |
