| 摘要: |
| 建立黄花蒿中青蒿素的高效液相色谱测定方法,采用柱前衍生RP-HPLC法测定黄花蒿中青蒿素的含量,采用ZORBAXXDB-C18(4.6 mm×150 mm,5 μm)色谱柱,甲醇-0.01 mol/L醋酸钠-醋酸缓冲液(pH5.8,体积比62:38)为流动相; 检测波长:260 nm; 流速:0.8 mL/min; 柱温:30 ℃。对广西、沈阳、北京、郑州、苏州和杭州等不同产地的野生黄花蒿样品、以及同一产地不同采集时间黄花蒿样品进行检测,结果表明不同地区青蒿素含量差异很大,同一地区不同采集时间黄花蒿样品的青蒿素含量也有差异。该法准确可靠、重现性好,能准确地反映青蒿素含量的检测。 |
| 关键词: RP-HPLC 黄花蒿 青蒿素 含量测定 |
| DOI： |
| 分类号:Q946 |
| Fund project: |
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| Determination of artemisimin in Artemisia annua with RP-HPLC |
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LIU Jin-Lei, LI Dian-Peng*, WEI Xiao, HUANG Yong-Lin,
LU Feng-Lai, JIANG Yun-Sheng
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Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
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| Abstract: |
| The content of artemisimin in Artemidia annua was determined with the method of pre-column reaction to RP-HLPC. The column was ZORBAXXDB-C18(4.6 mm×150 mm,5 μm),with mobile phase CH3OH-0.01 mol/L NaAC-HAC buffer(pH=5.8,62:38),flow rate 0.8 mL/min,column temperature at 30 ℃. The results showed that there are great diversity in the content of artemisimin in Artemidiae annua from different places and different harvest time. The method is accurate,sensible and repeatable,and can be used for the quality control of this herbal medicine. |
| Key words: RP- HPLC Artemisia annua L. artemisimin determination |
