| 摘要: |
| 马卡属于十字花科独行菜属,具有极高的营养价值和药用价值。在快繁过程中,光对马卡愈伤组织生长,丛生芽的诱导和存活有显著的影响。绿光和蓝光既不利于愈伤组织的生长也不利于丛生芽的诱导和存活。白光、红光和黄光能明显促进愈伤组织的生长,在这些光照条件下丛生芽的诱导率为60%～80%,丛生芽存活率为29%～36%。适当延长光照时间可提高丛生芽的存活率,合适的光照时间为16 h/d。但是过强的光照可使丛生芽的存活率降低,合适的光照强度为24～41 μmol/m2·s。 |
| 关键词: 光质 光周期 光强 马卡 愈伤组织 丛生芽 |
| DOI： |
| 分类号:Q813.1 |
| Fund project: |
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| Effects of light on maca callus growth, shoot induction and its survival rate |
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Wang Ya-Li1,2, Wang Xiao-Dong1, Zhao Bing1, Wang Yu-Chun1*
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1.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences,
Beijing 100080, China;2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Maca(Lepidium meyenii),belongs to the family of Brassicaceae,has high value of nutrition and medicine. In the process of micropropagation,light had remarkable influences on maca callus growth,shoot induction and survival. Green light and blue light not only made against maca callus growth but also inhibited shoot induction and survival. White light,red light and yellow light improved callus growth remarkably. Under these light conditions,the shoot induction rates were from 60% to 80%,and the shoot survival rates were from 29% to 36%.Appropriate prolonging of the time under light increased the shoot induction rate,and the appropriate photoperiod was 16h per day. But higher intensity of light decreased the survival rate of the shoots,and the appropriate light intensity was 24-41 μmol/m2·s. |
| Key words: spectral quality photoperiod light intensity maca callus shoot |
