| 摘要: |
| 为了探究云锦杜鹃的挥发性成分,该研究采用顶空固相微萃取-气相色谱-质谱联用技术对云锦杜鹃不同花期中的挥发性成分进行定性定量分析,并通过主成分分析法分析其特征挥发性成分。结果表明:四个花期共检测出50种挥发性成分,共分为苯丙酸类/苯环型、萜烯类、醇类、醛类、烃类和其他类六大类组分。对29种主要挥发性物质进行主成分分析,提取了两个主成分,累计方差贡献率达到88.545%。分析发现,β-月桂烯、β-罗勒烯、可巴烯、异喇叭烯、桉树脑、 衣兰烯、(+)-表二环倍半水芹烯、(3R-反式)-4-乙烯基-4-甲基-3-(1-甲基乙烯基)-1-(1-甲基乙基)-环己烯与第1主成分呈高度正相关,第 2 主成分的贡献率为 31.455%,其中丁香酚的影响最大,呈高度负相关,这些物质是影响云锦杜鹃香气的关键性成分。在9种高度相关的物质中,萜烯类物质占了7种。综上认为,萜烯类物质是云锦杜鹃主要特征香气成分。 |
| 关键词: 云锦杜鹃, 不同花期, 挥发性成分, 主成分分析 |
| DOI：10.11931/guihaia.gxzw201905030 |
| 分类号:Q946 |
| 文章编号:1000-3142(2020)07-1033-13 |
| Fund project:浙江省一流学科“生物工程”学生创新项目(CX2018015); 浙江省大学生科技创新活动计划暨新苗人才计划项目(2018R420022); 浙江省重中之重学科生物工程开放基金(1740002923); 宁波市科技重大专项项目(2014C11002)[Supported by Zhejiang Province's First-Class Discipline “Bioengineering” Student Innovation Project(CX2018015); Zhejiang University Students' Science and Technology Innovation Activity Plan(2018R420022); Zhejiang Provincial Key Discipline Bioengineering Open Fund(1740002923); Ningbo Science and Technology Major Project(2014C11002)]。 |
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| Analysis of volatile components of Rhododendron fortunei at different flowering stages by HS-SPME-GC-MS and PCA |
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ZHANG Chenfei, XIE Xiaohong, WANG Qinghao, WANG Wenjing,
WANG Jinyang, XIE Yu, WU Yueyan*
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College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China
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| Abstract: |
| This study aimed to determine the volatile components emitted of Rhododendron fortunei in different flowering stages by headspace solid phase microextraction-gas chromatograhymass spectrometry(GC-MS)as well as principal component analysis(PCA). A total of fifty volatile components were detected throughout the whole flowering stages, which were mainly phenylproanoids/benzenoids, terpenoids, alcohols, aldehydes, hydrocarbons and others. The principal component analysis of twenty-nine major volatile components was carried out, and the cumulative bution rate of the two principal components was 88.545%. β-myrcene, β-ocimene, copaene, isoledene, eucalyptol, ylangene, epi-bicyclosesquiphellandrene, cyclohexene, 4-ethenyl-4- methyl-3-(1-methylethenyl)-1-(1methylethyl)-,(3R-trans)- were highly positively correlated with PC1. The contribution rate of the second principal component is 31.455%, and the effect of Eugenol is the largest and highly negatively correlated. These components are the key aroma components of Rhododendron fortunei flowers. Of the nine highly related components, terpenes accounted for seven species. Therefore, the terpenoids were the major aromas constituents for Rhododendron fortunei. |
| Key words: Rhododendron fortunei,different flowering stages,volatile component,principal component analysis(PCA) |
