巴戟天种质离体保存研究

Study on preservation in vitro of Morinda officinalis

关键词:  巴戟天  离体保存  生长调节剂  CCC  PP₃₃₃  ABA  MH

Key words:  Morinda officinalis  preservation in vitro  plant regulator  CCC  PP₃₃₃  ABA  MH

摘要:

以巴戟天试管苗为材料,研究不同浓度的无机盐、蔗糖和四种植物生长调节剂(CCC、PP₃₃₃、ABA、MH)对巴戟天试管苗保存的影响。结果表明:巴戟天试管苗在无生长调节剂的MS、1/2MS、1/4MS三种无机盐浓度培养基上均能保存培养360 d,存活率达90%; 蔗糖浓度为20～40 g/L时,植株生长健壮,能延长保存时间; 四种生长调节剂均能诱导试管苗侧芽的生成,抑制顶芽和叶片的生长。其中以PP₃₃₃促进壮苗效果最好,能提高试管苗素质,在1/2MS+PP₃₃₃0.5～1.0 mg/L+蔗糖30 g/L培养基上,试管苗能保存480 d,存活率达100%。

Abstract:

Effects of the mineral nutrition,sucrose and plant regulator(CCC,PP₃₃₃,ABA,MH)in different concentrations on preservation in vitro of Morinda officinalis were examined. The results showed that tube plantlets of Morinda officinalis can be preserved for 360 d on MS,1/2MS and 1/4MS medium,with survival rate of 90%. When the concentration of sucrose in the medium was 20～40 g/L,the plantlets grown well and could still be longly preserved. Their lateral buds were induced while the growth of top buds and leaves were inhibited by these regulators. Medium with PP₃₃₃ was good for producing intact vigorous plantlets. On PP₃₃₃0.5～1.0 mg/L+1/2MS,the tube plantlets could be conserved for 480 d with 100% survival rate.