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| 辣椒CaNPR1-RNAi表达载体的
构建及其对辣椒的转化 |
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罗 欢1,2, 段承杰1,2, 陈保善1,2, 唐纪良1,2, 冯家勋1,2*
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1.广西亚热带生物资源保护利用重点实验室, 南宁 530005;2.广西大学 生命科学与技术学院, 南宁 530005
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| 摘要: |
| 从辣椒中克隆到一个与拟南芥系统获得抗性正调节基因NPR1同源的CaNPR1基因的全长cDNA。辣椒CaNPR1基因与拟南芥NPR1基因在mRNA水平上同源性为62.9%,两者的编码产物一致性为49.7%,相似性为65.7%。为鉴定该基因的功能,构建了一个以CaNPR1基因为靶基因的RNA干涉辣椒表达载体pCaNPR1-RNAi,并用根癌农杆菌介导法转化辣椒桂研五号,共获得了6株卡那霉素抗性再生苗,经Southern杂交证实,这些再生苗均为转基因植株,为进一步鉴定该基因的功能奠定了基础。 |
| 关键词: CaNPR1基因 克隆 RNA干涉 辣椒遗传转化 |
| DOI： |
| 分类号:Q943 |
| Fund project: |
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| Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper |
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LUO Huan1,2, DUAN Cheng-Jie1,2, CHEN Bao-Shan1,2
TANG Ji-Liang1,2, FENG Jia-Xun1,2*
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1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning
530005, China;2.College of Life Science and Technology, Guangxi University, Nanning 530005, China
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| Abstract: |
| Full-length cDNA of CaNPR1,a homologous gene of the positive regulatory gene NPR1 in systemic acquired resistance of Arabidopsis thaliana,was cloned from pepper(Capsicum annuum). CaNPR1 and NPR1 share 62.9% homology at mRNA level and their encoded products have 49.7% identity and 65.7% similarity. In order to identify the function of CaNPR1 in SAR of pepper, an expression vector pCaNPR1-RNAi for silencing CaNPR1 in pepper was constructed. Pepper cultivar Guiyan5 was transformed with pCaNPR1-RNAi by Agrobacterium tumefaciens-mediated transformation. Six kanamycin-resistant transgenic seedlings were obtained. Southern analysis confirmed that all the six seedlings carried the introduced transgene. |
| Key words: CaNPR1 gene cloning RNA interference pepper transformation |
