冰冻切片法在植物微管骨架研究中的应用

Cryosectioning method for the observation of microtubule cytoskeleton in plant cells

关键词:  冰冻切片法  植物微管骨架  荧光显微镜  免疫荧光标记  甘蔗

Key words:  cryosectioning method  plant microtubule cytoskeleton  fluorescence microscopy  Immunofluorescence staining  sugarcane(Saccharum officinarum)

摘要:

介绍了冰冻切片法研究植物微管骨架的一般程序和技术上的一些改进,结果证明,改进的冰冻切片技术,可以对植物不同类型的细胞进行很好的标记。实验结果表明,甘蔗正在迅速伸长的幼叶分布的微管类型主要是与细胞伸长轴方向垂直的周质微管,幼叶基部尤其是第三幼叶基部分布的主要是与细胞伸长轴方向平行的周质微管。表明冰冻切片法在植物微管骨架的研究中具有广阔的应用前景。

Abstract:

Plant Microtubule cytoskeletons have long been known to play a key role in plant morphogenesis. The general procedures and some improvements of cryosectioning method for the plant microtubule were detailed here. After fixation,microtubule arrays in various cells could be stained by using improved cryosection method. Transverse cortical microtubules to the axis of cells were the main microtubule arrays in the elongating young leaves,and longitudinal arrays to the long axis of the cells in the primordium,especially at the base of the third young leaf. The results indicate that the cryosectioning method is useful for investigating plant microtubule cytoskeleton.