茅苍术内生真菌的分离鉴定及在组培苗中的回接

Endophytic fungi screening from Atracty lancea and inoculating into the host plantlet

关键词:  茅苍术  组织培养苗  内生真菌  炼苗  抗逆

Key words:  Atracty lancea  tissue rapid propagation plantlets  endophytic fungi  domestication  adversity tolerance

摘要:

从茅苍术的叶、茎和根中分离鉴定内生真菌,并观测内生菌回接对茅苍术组培苗生长的影响。共获得茅苍术内生菌16株,其中叶片中14株,根茎中各获得一株。分别为交链孢霉、镰刀霉、小克银汉霉、青霉、茎点霉、小菌核菌、孔球孢霉和不产孢类。经内生菌和茅苍术快繁苗实验初筛,得到两株对植物无害的内生菌:小菌核菌和孔球孢霉,将它们回接到茅苍术组培苗中,可以从苗叶片中染色观察到菌丝。其中小菌核菌能提高茅苍术炼苗的存活率,接入内生菌的茅苍术叶片超氧化物歧化酶和过氧化物酶活性均高于未接菌的对照,脂肪酸不饱和指数基本不变,说明分离自茅苍术的内生菌回接后可以与宿主建立共生关系。

Abstract:

The endophytic fungi were screened and identified from Atracty lancea. Sixteen strains of endophytic fungi were screened totally,fourteen from leaves. One strain was from root and stem respectively. These strains including Alternaria sp.,Fusariium sp.,Cunninghamella sp.,Penicillum sp.,Phoma sp.,Sclerotium sp.,Gilmaniella sp.and Myceliasterilia. One strain of Sclerotium and one strain of Gilmaniella which no harm to plant tested by culturing together with tissue rapid propagation plantlets were inoculated into propagation plantlets. The influence of the endophytic fungi on the growth,livability and physiology of A.lancea were studied. Sclerotium sp.can strongly increase the livability of A.lancea. The mycelium could be observed by dying the material. Superoxide dismutase and peroxidase activilities of treated groups were both higher than that of control. Index of unsaturated fatty acid was the same as that of control. These were consistent with the result that endophytic fungi were symbiosis with the host.