| 摘要: |
| 根据提取RNA质量等方面比较了4种RNA提取方法提取东亚砂藓正常生长和干燥处理后的组织的总RNA的效果。结果表明改良的SDS法能提取高质量的RNA。该方法还可用于紫萼藓等藓类植物RNA的提取。 |
| 关键词: 藓类 东亚砂藓 RNA 提取 |
| DOI： |
| 分类号:Q94-3 |
| Fund project: |
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| Study on extraction methods of bryophytes genomic RNA |
|
YAN Miao-Miao1, WEI Guang-Cheng1, SHA Wei2*, Lü Feng-Xiang2
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1.Department of Basic Medical Science, Binzhou Medical College, Yantai 264003, China;2.Department of
Biology, College of Life Science and Engineering, Qiqihar University, Qiqihar 161006, China
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| Abstract: |
| 4 methods for RNA extraction from normal and dried Racomitrium japonicum tissues were evaluated upon time consumption,yield and quality of RNA isolation. A modified SDS method was found to be the best one among the 4 methods evaluated. The method was found to be also suitable for extracting RNA from bryophytes such as Grimmia pilifera etc. |
| Key words: moss Racomitrium japonicum RNA extraction |
