| 摘要: |
| 以外源茉莉酸甲酯(JA-Me)处理拟南芥,运用膜片钳技术研究JA-Me、过氧化氢(H2O2)和内向K+通道之间的关系,以探讨茉莉酸类物质(JAs)抑制根伸长生长分子机制。检测到10-4 mol/L的JA-Me能抑制根细胞质膜内向K+电流,表明可能与根的伸长生长有关,并且发现H2O2可能作为第二信使参与了JAs抑制根伸长生长的过程,H2O2介导的JA-Me对根细胞内向K+通道的抑制是根生长受抑的可能电生理机制。 |
| 关键词: JA-Me 根伸长生长 内向K+通道 H2O2 拟南芥 |
| DOI： |
| 分类号:Q945.3 |
| Fund project: |
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| Electrophysiological mechanism of root length growth of Arabidopsis thaliana inhibition by JA-Me |
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LI Huan-Qing1,2, LI Gui-Ling1, CUI Xiang-Huan2,
AN Guo-Yong2, SONG Chun-Peng2
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1.College of Bioengineering, Henan University of Technology, Zhengzhou 450052, China;2.College of Life Sciences, Henan University, Kaifeng 475001, China
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| Abstract: |
| Arabidopsis thaliana(ecotype Columbia)was treated with methyl-jasmonate(JA-Me)and the relations of JA-Me,H2O2 and inward potassium channel were examined by using patch clamp technique. The results showed that different concentrations of JA-Me(10-8-10-3mol/L)could all inhibit the elongation of primary roots to some degree. The inward K+ currents of root cortical protoplasts were inhibited with 10-4 mol/L JA-Me and 10-5 mol/L H2O2 treatment. What's more,10-4 mol/L DPI and 10-2 mol/L Vc could both reverse the JA-Me-inhibited inward K+ currents respectively,which suggested that H2O2 may act as a second messenger involving JA-Me inhibition of the root inward K+ currents. The results indicate that JA-Me inhibition of root length growth may result from the H2O2 induced JA-Me inhibition of the root inward potassium channel. |
| Key words: methyl-jasmonate root length growth inward potassium channel hydrogen peroxide Arabidopsis thaliana |
