拟南芥植物硫肽激素基因在玫瑰茄细胞中的表达

Expression of AtPSK2 gene in roselle cells

关键词:  拟南芥植物硫肽激素基因  玫瑰茄  根癌农杆菌介导的遗传转化系统  转基因细胞  植物细胞增殖

Key words:  AtPSK  Roselle(Hibiscus sabdaiffa)  Agrobacterium tumefaciens-mediated genetic transformation system  transgenic cell  plant cell proliferation

摘要:

利用玫瑰茄愈伤组织遗传转化体系,通过根癌农杆菌将AtPSK2基因导入玫瑰茄细胞,并获得了功能性表达。研究结果表明,所获得的转基因玫瑰茄细胞系的临界植板细胞密度和临界起始细胞密度分别降低了60%和50%,悬浮细胞培养周期由16 d缩短到12 d,转化愈伤组织的比生长速率比对照提高了75%,转基因玫瑰茄细胞和愈伤组织中的花黄素含量与对照相比没有发生明显变化。本研究所获得的转基因玫瑰茄细胞系不仅为花青苷和花黄素等次生代谢产物高产细胞株的选育提供出发材料,而且为PSK-α作用机理的深入研究提供一个有用的模型。

Abstract:

By using A.tumefaciens-mediated genetic transformation system of Roselle callus,AtPSK2 gene was introduced into and expressed functionally in Roselle cells. The results demonstrated that,by comparison with un-transgenic Roselle cell or callus,the critical plating cell density and critical initiating cell density of transgenic cell line were cut down 60% and 50%,respectively,the growth cycle of transgenic cell in suspension culture was shortened from 16 days to 12 days,and the specific growth rate of transgenic Roselle callus was raised 75%,the content of flavonoid compounds in transgenic Roselle cell line or callus was hardly altered. The obtained transgenic Roselle cell line in this study not only provides an initial material for selection and breeding of Roselle cell line with high-yield anthocyanin,but also gives a useful research model for further investigation in the mechanism of PSK-α action.