| 摘要: |
| 综述了离体培养、有利基因的克隆及遗传转化等生物技术的三个方面在荔枝研究中的应用进展。已有研究者分别建立了花粉培养、叶片培养、幼胚培养等获得植株的再生体系,克隆了部分荔枝基因,建立了基因枪与根癌农杆菌转化体系。这些研究为荔枝生物技术育种提供了基础。 |
| 关键词: 荔枝 生物技术 离体培养 基因克隆 遗传转化 |
| DOI： |
| 分类号:Q813 |
| Fund project: |
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| Advances in biotechnological research on Litchi(Litchi chinensis) |
|
WANG Jia-Bao*, JIA Cai-Hong, XU Bi-Yu,
JIN Zhi-Qiang, LI Mei-Ying
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Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
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| Abstract: |
| The advances in research on in vitro culture,gene cloning and genetic transformation of litchi were reviewed in this paper. Plant regeneration from pollen,leaves and young embryo had already been succeeded. Some useful genes had been cloned and the genetic transformation mediated by particle bombardment and Agrobacterium tumefaciens infection had been constructed. The problems and prospects of litchi biotechnology were also discussed. |
| Key words: litchi biotechnology in vitro culture gene cloning genetic transformation |
