三种百合鳞茎提取物的抑菌作用

An evaluation on the antibacterial effects of the bulb extract from three Lilium species

关键词:  百合鳞茎  提取物  抑菌作用

Key words:  lily bulb  extracts  antibacterial effect

摘要:

采用纸片琼脂扩散法测定了宜昌百合、岷江百合及兰州百合鳞茎甲醇提取物对革兰氏阳性细菌(金黄色葡萄球菌、枯草芽孢杆菌)和革兰氏阴性细菌(大肠杆菌、沙门氏菌)的抑制活性,并对3种百合鳞茎提取物的含量与抑菌活性进行了剂量-效应关系分析。结果表明:3种百合鳞茎提取物对4种细菌均具有抑制活性,对革兰氏阳性细菌的抑菌活性高于对革兰氏阴性细菌的抑菌活性,且宜昌百合和岷江百合两种野生百合鳞茎提取物的抑菌活性均高于普通食用的兰州百合; 3种百合鳞茎提取物的含量与抑菌活性之间存在明显的剂量-效应关系,即随着提取物含量的升高,抑菌活性明显升高。

Abstract:

Kirby-Bauer disk agar diffusion method was used to determine the antibacterial activities of the bulb extracts from Lilium leucanthum,L.regale and L.davidii to Gram-positive bacteria(Staphylococcus aureus,Bacillus subtlis)and Gram-negative bacteria(Escherichia coli,Salmonella),and the dose-effect relation between the contents of lily bulb extracts from three different species and the antibacterial activity was measured. The results showed that the lily bulb extracts from three Lilium species had antibacterial effects on all four tested bacteria,and that the effect on Gram-positive bacteria was stronger than that on Gram-negative bacteria. The inhibitory activities of L.leucanthum and L.regale extracts were higher than that of L.davidii. There existed a significant dose-effect relation between the antibacterial activity and the contents of lily bulb extracts,i.e.the antibacterial activity increased proportionally along with the increasing of the contents of lily bulb extracts.