鄂尔多斯半日花提取物的杀线虫作用

Nematicidal activity of extracts of Helianthemum ordosicum against Bursaphelenchus xylophilus

关键词:  鄂尔多斯半日花  松材线虫  三因素分析  最佳处理组合  LC₅₀

Key words:  Helianthemum ordosicum  Bursaphelenchus xylophilus  analysis of three factors  the optimal treatment combination  LC₅₀

摘要:

为全面了解濒危植物半日花的价值,测定并比较半日花不同浓度的6种提取物药液对松材线虫的生物活性,选出有较强杀线虫活性的甲醇、乙醇及水提取物,系统研究了提取物种类、浓度和处理时间对松材线虫死亡率的影响。三因素方差分析表明,提取物种类(A)、处理浓度(B)和处理时间(C),三因素的互作效应(A×B×C)和双因素互作效应(A×B、A×C、B×C)对线虫的校正死亡率都有极显著影响。最佳药液处理为:水提取物在6 mg·L^-1浓度时与1 d的作用组合和乙醇提取物在3 mg·L^-1浓度时与5 d的组合,以上两种组合的校正死亡率均达到100%。比较水、乙醇提取物5 d中的LC₅₀值,确定水是提取活性物质的首选溶剂。

Abstract:

To found out the value of Helianthemum ordosicum,the biological activities of six kinds of extract on different concentrations were determined and compared. And the carbinol,ethanol and water extracts which have strong nematicidal activity were selected from those extracts. The influences of extract types,treatment concentrations and treatment time on Bursaphelenchus xylophilus were investigated. Variance analysis of three factors showed Helianthemum ordosicum's corrected mortality was extremely influenced by type(A),treatment concentration(B)and treatment time(C),interaction effect of three factors(A×B×C)and interaction effects between factors(A×B,A×C,B×C)had the same effect. The optimal treatment combinations were solved: one was the water extract 6 mg·L^-1 on the 1st day; the other was ethanol extract 3 mg·L^-1 on the 5th day,the Helianthemum ordosicum's corrected mortalities of the above two combinations were all 100%. The water and carbinol extract's LC₅₀ in 5 days were compared,and the nematocidal activity of water extract were significantly stronger than others.