摘要: |
在长期叶表皮制片过程中发现,过氧化氢-醋酸离析法简便实用,对蕨类植物小型叶和大型叶、裸子植物鳞形叶、钻形叶、针形叶、条形或带形叶及被子植物宽大的叶片均能适用。尤其重要的是,过氧化氢-醋酸离析法不会破坏叶表皮细胞的结构,有利于精确观察叶表皮形态特征,是一种比较理想的叶表皮离析方法。 |
关键词: 叶表皮 离析方法 过氧化氢-醋酸 |
DOI: |
分类号:Q944.5 |
Fund project: |
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H2O2-CH2COOH maceration——a simple and perfect method for leaf epidermis |
SUN Tong-Xing1, JIANG Xing-Shan2
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1.School of Life Science and Technology, Yancheng Teachers College, Yancheng 224002, China;2.College of Life Science, South China Agricultural University, Guangzhou 510642, China
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Abstract: |
The characteristics of H2O2-CH2COOH maceration for the preparation of leaf epidermis was reviewed. The maceration in equal volumes of acetic acid and 30% hydrogen peroxide didn't destroy the structures of epidermal cells and was suitable for the leaf epidermis of pteridophyte,gymnosperms and angiosperms. |
Key words: leaf epidermis maceration hydrogen peroxide and acetic acid |