| 摘要: |
| 从大豆冀nf37和冀豆15中克隆了大豆球蛋白G1基因的启动子片段。序列分析表明,两种启动子片段均为688 bp,与GenBank现有的3种启动子序列(四川大豆(DQ250808)、南农87-c38(AY649096)和Dare(X15121))间的同源性在96.4%～99.6%之间。其中来自冀nf37的启动子片段除Legumin盒上有一个碱基差异外,其它元件与DQ250808完全相同,据此推测该启动子片段具有种子特异性启动子活性。将其与已有γ-生育酚甲基转移酶基因连接,构建了种子特异性表达载体pBG1TMT,为通过代谢工程手段调控油料作物种子维生素E组成、提高其营养品质奠定了基础。 |
| 关键词: 大豆 球蛋白G1 启动子 γ-生育酚甲基转移酶(γ-TMT) 种子特异性表达载体 |
| DOI： |
| 分类号:Q943 |
| Fund project: |
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| Cloning of soybean glycinin G1 promoter and construction of plant expression vector |
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WANG Yong-Qin, CHEN De-Fu, WANG Hui-Zhuan, CHEN Xi-Wen*
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College of Life Sciences, Nankai University, Tianjin 300071, China
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| Abstract: |
| A fragment of glycinin G1 promoter was amplified from soybean cultivars Ji nf37 and Jidou 15,respectively. Sequence analysis indicated that both the fragments are 688 bp and had 96.4%-99.6% homologies compared with the registered sequences in GenBank(Sichuan(DQ250808),Nannong87-c38(AY649096)and Dare(X15121)). The promoter fragment from Ji nf37 contains the completely identical elements for seed-specific expression with DQ250808 except only one base difference existing in the Legumin box,which was supposed to have the activity of seed-specific promoter. It was recombined with the γ-TMT gene and plant expression vector pBG1TMT was constructed. The work laid foundation for metabolic engineering to increase α-tocopherol level in oilseed crop seeds. |
| Key words: soybean glycinin G1 promoter γ-tocopherol methyltransferase(γ-TMT) seed-specific expression vector |
