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欧美107杨树提取物体外对植物病原真菌的抑制活性
周亚明 刘 浩 钟灵允 周立刚 隋 鹏 郭泽建
中国农业大学 农学与生物技术学院, 北京 100193
摘要:
制备了欧美107杨树枝条和叶的乙醇粗提物及不同极性溶剂的萃取部分,并测定了它们对植物病原真菌的抑制活性。枝条和叶的乙醇粗提物对棉花枯萎病菌、小麦纹枯病菌、番茄早疫病菌、番茄枯萎病菌、黄瓜枯萎病菌、小麦赤霉以及玉米弯孢等7种植物病原真菌均具有一定的抑制作用。而枝条的乙醇粗提物对杨树溃疡病菌的菌丝生长有一定的促进作用。抗菌活性成分主要集中在乙酸乙酯萃取部分
关键词:  杨树  提取物  植物病原真菌  抗真菌活性  乙酸乙酯萃取部分
DOI:
分类号:S792.11; Q946.887
Fund project:
Inhibitory activity of extracts and fractions of Populus ×euramericana on plant pathogenic fungi in vitro
ZHOU Ya-Ming, LIU Hao, ZHONG Ling-Yun, ZHOU Li-Gang*, SUI Peng, GUO Ze-Jian
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Abstract:
The ethanol extracts and their different polar fractions from both young stems and leaves of Populus×euramericana Guineir cl. ‘74/76'were screened for antifungal activity against plant pathogenic fungi by using a mycelial radial growth inhibition method. The pathogenic fungi tested were Alternaria solani,Curvularia lunata,Dothiorella gregaria,Fusarium graminearum,F.oxysporum f.sp.cucumerinum,F.oxysporum f.sp.lycopersici,F.vasinfectum and Rhizoctonia cerealis. All plant extracts and their fractions expressed antifungal activity except for the ethanol extract from young stems on Dothiorella gregaria,the pathogen of poplar stem rot. Ethyl acetate fraction showed higher antifungal activity than the others.
Key words:  poplar  extracts  plant pathogenic fungi  antifungal activity  ethyl acetate fraction
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